Fig. 4: KLF6 is involved in ART-induced apoptosis of WERI-Rb1 cells via the mitochondrial signaling pathway. | Cell Death & Disease

Fig. 4: KLF6 is involved in ART-induced apoptosis of WERI-Rb1 cells via the mitochondrial signaling pathway.

From: Artesunate induces mitochondria-mediated apoptosis of human retinoblastoma cells by upregulating Kruppel-like factor 6

Fig. 4: KLF6 is involved in ART-induced apoptosis of WERI-Rb1 cells via the mitochondrial signaling pathway.

a, b Mitochondrial Bax and Bcl-2 expression were detected and quantified, the Bcl2/Bax ratio was decreased after ART treatment. COXIV was used as a loading control. c, d Cytosolic cytochrome C expression was increased after ART treatment. β-Tubulin was used as a loading control. e ART promoted cleavage of caspases-9 and -3. β-Tubulin was used as a loading control. f, g Silencing of KLF6 significantly inhibited cleavage of caspases-9 and -3 detected by western blot and quantified. h Two caspase-specific inhibitors Z-LEHD-FMK and Z-DEVD-FMK reversed ART-induced apoptosis. All results are presented as the mean ± SD (n = 3, *P < 0.05, vs. control)

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