Fig. 5: MiR-194 targeting THBS1, STAT1, and LIF may be involved in promoting angiogenesis. | Cell Death & Disease

Fig. 5: MiR-194 targeting THBS1, STAT1, and LIF may be involved in promoting angiogenesis.

From: Hepatocyte-derived exosomal MiR-194 activates PMVECs and promotes angiogenesis in hepatopulmonary syndrome

Fig. 5

a Intersection of the TargetScan and AmiGO results. b Western blot analysis of THBS1, STAT1, and LIF expression in PMVECs incubated with conditioned medium, exosomes derived from HPS rats or transfected with miR-194 mimic, miR-194 inhibitor or both (n = 5). c Quantification of THBS1, STAT1, and LIF expression in PMVECs incubated with conditioned medium, exosomes derived from HPS rats or transfected with miR-194 mimic, miR-194 inhibitor, or both. d–f miR-194 inhibited the luciferase activity of reporter containing the wild-type but not mutant 3′-UTR of THBS1, STAT1, and LIF. Upper panel, miR-194 and its putative binding sequence in the 3′-UTR of THBS1, STAT1, and LIF. Mutation sequences are underlined. Lower panel, cells were co-transfected with NC or miR-194 mimics, pRL-TK and a firefly luciferase reporter plasmid carrying the wild-type (WT) or mutant (MUT) 3′-UTR. The luciferase activity of NC-transfectants from one of the experiments in the WT group was set as 1. pRL-TK that expressed Renilla luciferase was used to correct the difference in transfection and harvest efficiencies (n = 5). Data are presented as the mean ± SD. *p < 0.05, compared with NC control group; #p < 0.05, compared with anti-NC + exosome group. g–i Correlation of serum exosomal miR-194 and the expression of THBS1, STAT1, and LIF in PMVECs isolated from HPS rats (n = 20)

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