Fig. 5: Effect of TMEM45A inactivation on the recruitment of RAD51 to DNA damage foci.
From: Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin
SQD9 cells were transduced with lentiviral particles expressing shRNA control (shCTL) or shRNA targeting the mRNA of TMEM45A (shRNA 22). SQD9 cells were incubated with or without 100 mM of cisplatin in normoxic conditions for 4 h then fresh medium without cisplatin was added to the cells for 4 h. After the incubation, the recruitment of RAD51 was studied by confocal microscopy using anti-RAD51 antibody and Alexa 488 anti-rabbit antibody and the abundance of gH2AX was studied using anti-gH2AX antibody and Alexa 488 anti-rabbit antibody. The nucleus was detected with Hoechst. Scale bars: 50 μm. The RAD51 signal intensity was quantified by Image J on more than 100 cells. Results are expressed as mean ± SD (n = 3). *p < 0.05.
