Fig. 6 | Cell Death & Disease

Fig. 6

From: Inhibiting lysine 353 oxidation of GRP78 by a hypochlorous probe targeting endoplasmic reticulum promotes autophagy in cancer cells

Fig. 6

ZBM-H inhibited lung cancer cell growth and induced apoptosis. a, c Hypochlorous acid level determined in non-cancer cell and cancer cells. Human umbilical vein endothelial cells (HUVEC), A549 cells and Hela cells were incubated with ZBM-H (2 μM) for 30 min, and the change of fluorescence ratio was counted. Scale bar: 20 μm. b Morphological change of A549 cells treated with ZBM-H for indicated concentration. Scale bar: 20 μm. d A549 cell (treated with ZBM-H for 24 h) viability. e–g Western blot (WB) analysis of cleaved caspase-12 and BAX in A549 cells treated with ZBM-H at indicated concentrations for 24 h. glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as loading control. e, h WB analysis of cleaved poly ADP-ribose polymerase (PARP) in A549 cells treated with ZBM-H for 24 h. Data are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, n = 3

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