Fig. 4: OGD-treated neurons displayed enhanced internalization of EVs derived from HUVECs. | Cell Death & Disease

Fig. 4: OGD-treated neurons displayed enhanced internalization of EVs derived from HUVECs.

From: Neurons can upregulate Cav-1 to increase intake of endothelial cells-derived extracellular vesicles that attenuate apoptosis via miR-1290

Fig. 4

a HUVECs-derived EVs were labeled with DiI, and incubated with primary neurons. The internalization of DiI-EVs was recorded by live imaging, and shown at 0, 1, 2, and 3 h. b DiI-EVs were incubated with neurons from control group or OGD group for 12 h and observed under a fluorescence microscope. The mean fluorescence intensity (MFI) was determined to compare the difference of fluorescence intensity between the two groups (n = 3). c The level of miR-1290 in neurons incubated with HUVECs-derived EVs for 3 or 12 h was determined (n = 6). Bars = means ± s.e.m. *P < 0.05, ***P < 0.001.

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