Fig. 5: Cav-1 was significantly upregulated in neurons upon OGD treatment. | Cell Death & Disease

Fig. 5: Cav-1 was significantly upregulated in neurons upon OGD treatment.

From: Neurons can upregulate Cav-1 to increase intake of endothelial cells-derived extracellular vesicles that attenuate apoptosis via miR-1290

Fig. 5

a Primary neurons were treated with OGD for 1.5 h and incubated with HUVECs-derived EVs for 3 or 12 h. The mRNA levels of Cav-1, Flot-1, Pak-1, Rac-1, and Dyn-2 were determined using qRT-PCR. b, c Protein level of Cav-1 in neurons at 12 h in a was determined by Western blotting b and immunofluorescence c. d, e Mice were treated with MCAO-reperfusion. TTC staining was performed to show the ischemic regions (pale) d. Tissue lysates were prepared, and the protein level of Cav-1 in the damaged hemisphere and control side was determined using Western blotting e. Bars = means ± s.e.m., n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant.

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