Fig. 7: The interaction between internalized SynI antibodies and SynI decreases SV density and increases SynI liquid phase separation. | Cell Death & Disease

Fig. 7: The interaction between internalized SynI antibodies and SynI decreases SV density and increases SynI liquid phase separation.

From: Autoantibodies to synapsin I sequestrate synapsin I and alter synaptic function

Fig. 7

a, b Representative images (a) and frequency distribution (b) of GFP-SynI droplets incubated with either control CSF or LE-CSF (25 μg/ml) for 5 and 45 min. Scale bars, 10 and 0.05 μm for low and high magnification, respectively. Each condition was assessed by two independent incubations. c Representative micrograph showing synapses from primary hippocampal neurons incubated with either control CSF or LE-CSF (1,5 μg/ml) for 72 h. Scale bar, 300 nm. d Upper panels: Quantification of SV and docked SV density in the analyzed terminals. Lower panel: quantification of the spatial distribution of SVs from active zone (AZ). Graphs show means ± SEM, single neuron data points from three independent preparations **p < 0.01, Mann–Whitney test.

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