Fig. 4: Secreted factors from sNF96.2 cells modulate Capan-2 cell–cell interactions in a TGFβ-dependent manner.
From: Schwann cells support oncogenic potential of pancreatic cancer cells through TGFβ signaling
a Images of fluorescent Capan-2-nRFP+ cells cultured for 48 h either with Capan-2 CM or with sNF96.2 CM, and treated or not with SB-431542 TβRI inhibitor (IncuCyte™ Zoom imaging system). The black frames show representative areas of Capan-2 cells enlarged in the right-hand side of each image. Scale bars, 400 μm. b Proliferation assay of Capan-2-nRFP+ cells cultured for 72 h either with Capan-2 CM (white dot) or with sNF96.2 CM (black square) (automated live cell time-lapse counting using an IncuCyte™ Zoom imaging system). Quantification of Capan-2 cell proliferation is represented as mean ± SD (n = 3 independent experiments, significance is shown for the 72-h timepoint, ns: not significant). c Immunofluorescence detection of F-ACTIN (red) and β-CATENIN (green) proteins in Capan-2 cells located at the migratory front in a wound healing assay after 24 h, cultured either with Capan-2 CM or with sNF96.2 CM and treated or not with SB-431542. The white frames show representative areas that are enlarged in the upper right-hand corner of each image. White arrowheads in the insets show the plasmic membrane. Scale bars, 15 μm. CM conditioned medium.
