Fig. 8: Biological processes correlated with proteins more abundant in conditioned medium of sNF96.2 cells and Capan-2 cells cultured alone or in combination.

a Venn diagram showing the number of significantly differentially enriched proteins quantified (fold change >2; adjusted p-value < 0.05) in sNF96.2 CM and sNF96.2/Capan-2 CM compared to Capan-2 CM. b, c GO enrichment analysis of differentially secreted proteins in sNF96.2 CM (156 proteins) and in sNF96.2/Capan-2 CM (128 proteins), compared to Capan-2 CM. The figures show the top 20 GO biological processes grouped by enrichment (>2-fold) and p-value (<0.05). Motility, adhesion and ECM related-groups are represented in different colors. d Heatmap depicting the abundance levels (log10 normalized LFQ abundance values) of 47 individual proteins identified within motility, adhesion and ECM related-GO biological processes, in the three sNF96.2, Capan-2 and sNF96.2/Capan-2 CM. # are representative of a non-significant difference between sNF96.2 CM and Capan-2 CM. e Protein–protein predicted functional associations between 33 proteins enriched in sNF96.2 CM and sNF96.2/Capan-2 CM compared to Capan-2 CM and associated with motility, adhesion and ECM GO groups, are represented by a protein-protein network graph (STRING database analysis). The protein network is separated into 9 clusters (see node colors), in which inter-cluster edges are represented as dashed lines. Inter-node edges are representative both of types of action (color code: red, inhibition; blue, binding; purple, catalysis; black, reaction; green, activation; yellow, transcriptional regulation; gray, unspecified) and effects (unspecified, positive or negative) for predicted interactions between proteins. CM conditioned medium, ECM extracellular matrix, GO gene ontology, STRING search tool for the retrieval of interacting genes/proteins.