Fig. 1: In vitro screening of 18 patient-derived GBM cell cultures for response to JQ1 and TMZ.
From: BET and Aurora Kinase A inhibitors synergize against MYCN-positive human glioblastoma cells
JQ1 dose response curves were used to define JQ1-sensitive (a), JQ1-semi-sensitive (b) and JQ1-resistant class (c) of GBM cell lines. Unlike JQ1, TMZ successfully inhibited proliferation and viability of GBM cell lines used in this screen (d–f). When exposed to 500 nM JQ1 (EC50 concentration), 18 patient-derived GBM cell cultures exhibit differential viability response again showing three JQ1 response classes (g). Viability of assayed 18 GBM cell lines upon 500 nM JQ1 (EC50) inhibition significantly correlates (Pearson correlation) with viability upon 400μM TMZ inhibition (h). Classical and proneural GBM subtype dominated in the JQ1-sensitive class, while the JQ1-resistant class was represented by mainly mesenchymal GBM cell lines (i). CL classical, PN proneural, MS mesenchymal subtype.
