Fig. 2: Tumor growth of HCT116 cells and DAPK1 ko clones in the chicken CAM in vivo model.
From: DAPK1 loss triggers tumor invasion in colorectal tumor cells
a Scheme of a chicken CAM tumor assay. A albumen, AS amniotic sac, CAM chorioallantoic membrane, E chick embryo, S egg shell, SM shell membrane, T human tumor cells in Matrigel, YS yolk sac, dotted line invasion front of tumor cells investigated using pan-CK immunohistology. b Representative ex ovo images of HCT116 and DAPK1 ko clone derived xenograft tumors harvested 5 days post-implantation on the CAM of fertilized chicken eggs. C capillaries, T tumor, bv blood vessel, CAM chorioallantoic membrane. Scale bar = 3 mm. c Representative images of hematoxylin-eosin (HE) and d pan-cytokeratin (pan-CK) stained paraffin sections of CAM tumors (×20). Arrow head: tumor bud. Scale bar = 50 µm. e Average number of tumor buds (≤tumor 4 cells) counted on pan-CK stained sections across 4–10 high-power fields (HPFs; ×40). HCT116: n = 9; ko clones clone 7/6: n = 6; clone 10/8: n = 9; clone 21/9: n = 9; P = 0.06; Mann–Whitney test). f Pie chart diagram of high-score (≤1 mean bud number per up to 10 HPFs) and low-score (>1 buds) tumor budding. HCT116: n = 9 (nlow = 8; HCT116: nhigh = 1); DAPK1 ko clones: n = 24 (nlow = 12; nhigh = 12. g Percentage of intratumoral vessels counted on scans of HE stained sections expressed in %. HCT116: n = 9; DAPK1 ko clones: n = 24 (clone 7/6 n = 6; clone 10/8 n = 9; clone 21/9 n = 9). ***P ≤ 0.001 compared to HCT116 (Mann–Whitney test).
