Fig. 4: Enhanced metastatic capacity of DAPK1-deficient tumor cells.
From: DAPK1 loss triggers tumor invasion in colorectal tumor cells
a Light microscopy images of embedded 3D spheroids taken after 0 h, 48 h and 96 h of incubation. The manual selection of the spheroid area (red) is displayed in the upper left corner of each image. Pictures were taken at ×10 magnification. Scale −200 μm. b Quantification of the invasive potential of n 3D spheroids from two independent experiments (1. experiment: displayed in black; 2. experiment: displayed in blue). All values represent means ± S.D. and statistical analysis was performed using two-way ANOVA followed by Dunnett’s multiple comparisons test (HCT116 (●): n = 5/5; clone 7/6 (■): n = 5/5; clone 10/8 (▲): n = 5/5); clone 21/9 (◆): n = 5/5). (*P ≤ 0.05 and ***P ≤ 0.001). c HCT116 and DAPK1 ko cells were implanted on living organotypic brain slices. Areas of tumor cell invasion one and three days after implantation were analyzed. Representative images (×2.0) of fluorescence intensities of two independent experiments are shown. Relative tumor invasion was detected by mean fluorescence intensities (green). d one day (*P < 0.0368, **P < 0.0028 compared to HCT116) and (e) three days after implantation and presented as fold-change to HCT116. Black/gray dots present the first (n = 15), blue dots the second (HCT116: n = 14; clone 7/6: n = 14; clone 10/8: n = 14; clone 21/9: n = 15) experiment. f Optical overlay of representative surface images and fluorescence signal of deep-red fluorescence labeled HCT116 and clone 21/9 cells in chicken embryos 6 days after tumor cell implantation. Embryos with unstained tumor cell implants served as background control. The color bar indicates the range of average radiant efficiency ×107 from minimal (black) to maximal (yellow); Average radiant efficiency as detected by fluorescence imaging. B: brain; H: heart; L: lung." HCT116: n = 8; clone 21/9: n = 5. (**P < 0.0062; Mann–Whitney test).
