Fig. 8: ERK mediated signaling in DAPK ko clones.
From: DAPK1 loss triggers tumor invasion in colorectal tumor cells
a Representative images of phospho-ERK1/2 expression in CAM micro-tumor paraffin sections by immunohistochemistry. Blue boxes: ×2 zoomed in; scale bar = 50 µm. (b) Nuclear and (c) cytoplasmic immunoscore of phospho-ERK1/2 expression in CAM micro-tumors obtained by multiplying staining intensity with percentage positivity divided by 10 (HCT116: n = 6; clone 7/6: n = 7; clone 10/8: n = 4; clone 21/9: n = 4). A Mann–Whitney test was performed to calculate significance between two groups (*P = 0.021; ns P = 0.1539). d Western Blot analysis of pERKThr202/Tyr204, ERK1/2, PARP and cleaved PARP, ICAM1 and TACSTD2 protein expression in HCT116 cells treated with 0, 10, and 20 µM of ERK1/2 inhibitor (FR180204) for 48 h. Representative images are shown. GAPDH served as loading control. e Effects of siERK2 in HCT116, clone 21/9 and clone 7/6 cells on ERK/1/2, ICAM1 and TACSTD2 protein levels were investigated by Western Blot analysis in comparison to the corresponding non-treated and scrambled-treated cells. Cells were harvested 48 h after transfection. Representative images of two independent experiments are shown. GAPDH was used as loading control.
