Table 1 Fold change difference of mRNA expression of DAPK1 ko clones versus HCT116.

From: DAPK1 loss triggers tumor invasion in colorectal tumor cells

Reference sequence

Gene ID

Gene name

Average fold change (log2) ± SEM

P-value (t-test)

A: upregulated genes

NM_000641.2

IL11

Interleukin-11

5.91 ± 0.76

<0.001

NM_002353.2

TACSTD2

Tumor-associated calcium signal transducer 2

5.13 ± 3.12

0.004

NM_005127.2

CLEC2B

C-type lectin domain family 2 member B

4.71 ± 7.14

0.016

NM_018192.2

P3H2

Prolyl 3-hydroxylase 2

2.78 ± 0.64

0.007

NM_001105209.1

LAMA4

Laminin, alpha 4

2.31 ± 0.47

0.031

NM_002985.2

CCL5

Chemokine (C-C motif) ligand 5

2.25 ± 0.33

0.05

NM_012391.1

SPDEF

SAM pointed domain containing ets transcription factor

2.22 ± 0.43

0.031

NM_001143981.1

CHRDL1

Chordin-like 1

2.22 ± 0.36

0.011

NM_001165413.1

SERPINE1

Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

2.07 ± 0.34

0.001

NM_000093.3

COL5A1

Collagen, type V, alpha 1

1.95 ± 0.96

0.024

NM_020733.1

HEG1

Protein HEG homolog 1

1.78 ± 0.28

0.003

NM_198576.2

AGRN

Agrin

1.75 ± 0.16

<0.001

NM_003238.2

TGFB2

Transforming growth factor, beta 2

1.73 ± 0.36

0.012

NM_000094.2

COL7A1

Collagen, type VII, alpha 1

1.63 ± 0.85

0.037

NM_017423.2

GALNT7

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)

1.57 ± 0.03

<0.001

NM_001005376.1

PLAUR

Plasminogen activator, urokinase receptor

1.57 ± 0.10

<0.001

NM_020770.2

CGN

Cingulin

1.57 ± 0.32

0.034

NM_181501.1

ITGA1

Integrin, alpha 1

1.56 ± 0.32

0.049

NM_017617.3

NOTCH1

Neurogenic locus notch homolog protein 1

1.55 ± 0.17

0.021

NM_001060.3

TBXA2R

Thromboxane A2 receptor

1.52 ± 0.21

0.038

NM_005560.3

LAMA5

Laminin subunit alpha-5

1.5 ± 0.17

0.006

NM_022648.4

TNS1

Tensin 1

1.5 ± 0.19

0.011

B: down-regulated genes

NM_000214.2

JAG1

Jagged 1

−1.58 ± 0.34

0.035

NM_005795.3

CALCRL

Calcitonin receptor-like

−1.62 ± 0.04

0.006

NM_032777.9

GPR124

G protein-coupled receptor 124

−1.73 ± 0.13

0.023

NM_003469.3

SCG2

Secretogranin II

−2.2 ± 1.13

0.043

NM_031479.3

INHBE

Inhibin, beta E

−2.28 ± 0.62

0.016

NM_014333.3

CADM1

Cell adhesion molecule 1

−2.42 ± 0.57

0.049

NM_000020.1

ACVRL1

Activin A receptor type II-like 1

−2.53 ± 0.40

0.009

NM_004369.3

COL6A3

Collagen, type VI, alpha 3

−2.62 ± 0.57

0.004

NM_002010.2

FGF9

Fibroblast growth factor 9

−2.88 ± 1.78

0.042

NM_000029.3

AGT

Angiotensinogen (serpin peptidase inhibitor, clade A, member 8)

−2.88 ± 0.50

0.035

NM_001147.2

ANGPT2

Angiopoietin 2

−8.02 ± 1.44

0.002

NM_000201.2

ICAM1

Intercellular adhesion molecule 1

−9.94 ± 1.88

<0.001

  1. Gene expression analysis was performed using the human nCounter® PanCancer Progression Panel (NanoString Technologies, Hamburg, Germany). Only significant (P < 0.05) fold changes >−1.5 or > +1.5 fold were considered as differentially expressed in DAPK1 ko clones compared to the wildtype. Transcripts with an RNA count of <5 of all samples were excluded as they were considered as non-expressed. The average of clones (clone 7/6: n = 2; clone 10/8: n = 3; clone 21/9: n = 1) vs. HCT116 (n = 3) ± is presented. P value was calculated by nSolver software (Nanostring Technologies) using t-test. Expression profiling data are available online (GEO accession number: GSE130488)
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