Fig. 1: Doxorubicin or irinotecan significantly promoted KRT8 expression in chordoma cells in vitro. | Cell Death & Disease

Fig. 1: Doxorubicin or irinotecan significantly promoted KRT8 expression in chordoma cells in vitro.

From: Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy

Fig. 1

Chordoma cell line CM319 and UCH1 were being treated with doxorubicin (0.5 μM) or irinotecan (50 μM) for 12 h or 24 h. a KRT8 mRNA level was evaluated by qRT-PCR. b Western blotting analysis and quantification of KRT8 protein expression (normalized to GAPDH expression). c Representative images of immunofluorescence staining of KRT8 of CM318 and UCH1 cell line (n = 3, *p < 0.05 vs. control group, **p < 0.01 vs. control group, NS: not statistically significant vs control group, Con: control group, Doxo: doxorubicin-treated group, Irino: irinotecan-treated group. Scale bar = 50 μm. For all the above-mentioned statistical analyses, significance was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test and the results were shown as mean ± SD).

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