Fig. 2: Doxorubicin or irinotecan induced unfolded protein response in chordoma cells in vitro. | Cell Death & Disease

Fig. 2: Doxorubicin or irinotecan induced unfolded protein response in chordoma cells in vitro.

From: Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy

Fig. 2

 Chordoma cell lines CM319 and UCH1 were being treated with doxorubicin (0.5 μM) or irinotecan (50 μM) for 12 h or 24 h in vitro. a Splicing of XBP1 mRNA were evaluated by RT-PCR. b Western blotting analysis and quantification of CHOP, ATF4, ATF6, and BiP protein expression (normalized to GAPDH expression). c Representative images of immunofluorescence staining of CHOP and BiP of CM319 and UCH1 cell line (n = 3, *p < 0.05 vs. control group, **p < 0.01 vs. control group, NS: not statistically significant vs. control group, Con: control group, Doxo: doxorubicin-treated group, Irino: irinotecan-treated group. Scale bar = 50 μm. For all the above-mentioned statistical analyses, significance was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test and the results were shown as mean ± SD).

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