Fig. 4: Knockdown of KRT8 in chordoma cell increased its’ sensitivity to chemotherapy by promoting its apoptosis in vitro. | Cell Death & Disease

Fig. 4: Knockdown of KRT8 in chordoma cell increased its’ sensitivity to chemotherapy by promoting its apoptosis in vitro.

From: Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy

Fig. 4

Chordoma cell line CM319 and UCH1 were transfected with siKRT8 followed by treatment of doxorubicin (0.5 μM) or irinotecan (50 μM) for 24 h. a Cell viability of chordoma cells were determined by CCK8 assay. b Western blotting analysis and quantification of KRT8, cleaved PARP, and Caspase 4 protein expression (normalized to GAPDH expression, quantification data of KRT8 in the “Doxo 24 h” group and “Irino 24h” group were derived from the same data set in Fig. 1b). c Immunofluorescence staining of KRT8 of CM319 and UCH1 cell line. d Apoptosis of chordoma cells was determined by Annexin V-PE/PI staining measured by flow cytometry (n = 3, *p < 0.05 vs. indicated group, **p < 0.01 vs. indicated group, NS: not statistically significant vs. indicated group, Con: control group, Doxo: doxorubicin-treated group, Irino: irinotecan-treated group. Scale bar = 50 μm. For all the above-mentioned statistical analyses, significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test and the results were shown as mean ± S.D.).

Back to article page