Fig. 6: Rescuing effects of BID knockdown on BSB-treated HeLa cells.
From: BID and the α-bisabolol-triggered cell death program: converging on mitochondria and lysosomes
a ΔΨm dissipation after 5-h treatment with 40 μM BSB as assessed by JC-1 staining in flow cytometry. The decrease of JC-1 red fluorescence, typical of cells with depolarized mitochondria, is significantly more evident in control as compared to BID-knockdown cells. b Lysosomal destabilization after 5-h treatment with 40 μM BSB as evaluated by LTG-uptake assay. Shown is the shift from weak and diffuse cytoplasmic signal (disrupted lysosomes from control-siRNA cells) to a punctate pattern (intact lysosomes from BID-siRNA cells). Scale bar: 12 μm. c Expression of Beclin 1 in control and BID-knockdown cells after 16-h treatment with 80 μM BSB. Shown experiments are representative of 3. d Viability as assessed by MTT assay in cells transfected with control-siRNA or BID-siRNA and exposed to 10, 20, 30, 40, and 80 µM BSB for 96 h. Mean values and s.d. of six independent determinations were plotted.
