Fig. 3: Persistent hypoxia increases signaling molecules in pyroptosis pathway and enhances lactate dehydrogenase (LDH) in human primary trophoblasts. | Cell Death & Disease

Fig. 3: Persistent hypoxia increases signaling molecules in pyroptosis pathway and enhances lactate dehydrogenase (LDH) in human primary trophoblasts.

From: Pyroptosis is a critical inflammatory pathway in the placenta from early onset preeclampsia and in human trophoblasts exposed to hypoxia and endoplasmic reticulum stressors

Fig. 3: Persistent hypoxia increases signaling molecules in pyroptosis pathway and enhances lactate dehydrogenase (LDH) in human primary trophoblasts.

Primary trophoblast cells, isolated from the placentas from three deliveries with normal pregnancy at term, were treated with hypoxia (Hyp) or normoxia (Nor) for 3 days and lysed for immunoblotting. Hypoxia increases the expression of caspase-1, GSDMD, IL-18, IL-1β, and NLRP3. Comparison of the signal intensity reveals that these molecules are significantly elevated in hypoxia-treated primary trophoblasts relative to control cells (n = 3, p < 0.05). c LDH colorimetric assay shows higher level of LDH in supernatant from hypoxia-treated cells vs. control cells (n = 6, p < 0.05). Data are presented as mean ± SD and analyzed by a Student’s t test.

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