Fig. 1: Drosophila HP1a physically interacts with the MRN complex. | Cell Death & Disease

Fig. 1: Drosophila HP1a physically interacts with the MRN complex.

From: NBS1 interacts with HP1 to ensure genome integrity

Fig. 1

a Co-immunoprecipitation assay from HP1a-FLAG-overexpressing S2 cell extracts showing that HP1a precipitates endogenous Rad50, Mre11, and Nbs proteins (Input, 10% of total extract). The asterisk indicates aspecific bands. b, c Pulldown assays from Nbs-HA expressing Drosophila S2 cells with GST-tagged-full length HP1a (HP1a WT) and b GST-HP1aCSD and GST-HP1a∆CSD or c GST-HP1aW200A and GST-HP1I191E mutant proteins. Note that while Nbs has been revealed with a commercial anti-HA antibody, Rad50 and Mre11 have been detected with anti-Rad50- and anti-Mre11-specific antibodies generated in our laboratory. Ponceau staining shows the amount of each GST-tagged HP1a protein used in this assay. See text and “Materials and methods” for further details.

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