Fig. 2: Cav-1 expression is upregulated in L02 cells after AFB1 stimulation. | Cell Death & Disease

Fig. 2: Cav-1 expression is upregulated in L02 cells after AFB1 stimulation.

From: Critical role of caveolin-1 in aflatoxin B1-induced hepatotoxicity via the regulation of oxidation and autophagy

Fig. 2

L02 cells were treated with AFB1 (40 μM). The cells were collected at different time points. a Cav-1 mRNA levels were determined by RT-qPCR at different time points. b The protein levels of Cav-1 were examined in untreated (Ctrl) or AFB1-treated (40 μM) L02 cells via western blotting. GAPDH served as a control for equal sample loading. The level of Cav-1 was quantified by densitometric analysis using ImageJ software and normalized to GAPDH. c Localization of Cav-1. L02 cells were fixed following treatment with AFB1 (40 μM) for 36 h and were subjected to immunofluorescence to detect Cav-1 (green). The nuclei were stained with DAPI (blue). Images were obtained using a confocal microscope (scale bar = 20 μm). d The fluorescence intensity of Cav-1 was processed and quantified using ZEN Light Edition software. The data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.

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