Fig. 5: Role of Cav-1 in the regulation of Nrf2 transcriptional activity and the Keap1–Nrf2 interaction. | Cell Death & Disease

Fig. 5: Role of Cav-1 in the regulation of Nrf2 transcriptional activity and the Keap1–Nrf2 interaction.

From: Critical role of caveolin-1 in aflatoxin B1-induced hepatotoxicity via the regulation of oxidation and autophagy

Fig. 5

a L02 cells were untreated or treated with AFB1 (40 μM) for 36 h and collected samples were immunoprecipitated (IP) and immunoblotted (IB) with the indicated antibodies using the “Tag-Switch” method. Nonspecific IgG served as an input control. L02 cells transfected with NT, Cav-1 siRNA, Cav-1 plasmid, and the vector control were untreated or treated with AFB1 (40 μM) for 36 h, (b) cells were lysed and immunoblotted with anti-Keap1 antibodies, and (c) samples were collected for immunoprecipitation (IP) or western blotting (IB) analysis as indicated. The protein expression level was quantified by densitometric analysis using ImageJ software and normalized to GAPDH. The data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.

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