Fig. 1: Dot1l depletion in Tie2(+) cells causes lymphatic defects. | Cell Death & Disease

Fig. 1: Dot1l depletion in Tie2(+) cells causes lymphatic defects.

From: Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function

Fig. 1: Dot1l depletion in Tie2(+) cells causes lymphatic defects.

a, b Representative images of Dot1l∆EC and littermate control embryos at E13.5–15.5 and newborns. Scale bar = 2 mm. P1: postnatal day 1. c Immunohistochemistry analysis of E15.5 embryos. Images of Emcn (brown) and Lyve1 (red) in control and Dot1l∆EC mice. White rectangles represent enlarged images on right panels. Scale bar = 500 µm. Scale bar of the enlarged image = 100 µm. Arrow: skin edema. d Whole-mount confocal images stained with antibodies against Ter119 and Lyve1 in E15.5 sternum. Scale bar = 100 µm. e Representative whole-mount confocal images of E17.5 embryos stained with anti-Lyve1 and anti-Emcn antibodies. Lyve1-positive dots: macrophages. Scale bar = 200 µm. f Quantification of Lyve1(+) coverage in the mesenteries of E17.5 Dot1l∆EC (n = 11) and littermate control (n = 15) embryos.

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