Fig. 2: Dot1l loss impairs lymphatic valve formation. | Cell Death & Disease

Fig. 2: Dot1l loss impairs lymphatic valve formation.

From: Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function

Fig. 2: Dot1l loss impairs lymphatic valve formation.

a Gross overview of E17.5 embryos (upper panel, scale bar = 2 mm) and their whole-mount immunofluorescent staining with anti-Lyve1 and anti-Emcn antibodies in the respective mesenteries (lower panel) after a single TM injection (0.5 mg for E9.5 and 1.25 mg for E10.5 embryos). Scale bar = 200 µm. b Quantification of Lyve1(+) lymphatic coverage in TM-injected E17.5 Dot1liKO mesenteries (n = 3–16 embryos/group). c, d Representative immunofluorescence images and morphometric analysis of lymphatic valves in E17.5 Dot1liKO mesenteries after TM injection at E11.5. Green: Lyve1. Red: Prox1. Data are presented as mean ± s.e.m. *p-value ≤ 0.05.

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