Fig. 4: Dot1l function in c-Kit(+) HE is indispensable for the formation of mesenteric lymphatics.

a Representative images of E17.5 embryos (upper panel, scale bar = 2 mm) and whole-mount immunofluorescence staining of Dot1l∆HE mesenteries with anti-Lyve1 and anti-Emcn antibodies (lower panel, scale bar = 200 µm) after two consecutive 4-OHT injections. Incomplete lymphatic formation (arrows) is indicated. b Quantification of Lyve1(+) coverage in E17.5 Dot1l∆HE and control lymphatic vessels (n = 6–16 embryos per group).