Fig. 4: Nuclear translocation of GSK3β WT-GFP and mitochondrial Δψm dissipation at single cell level in vivo.
From: GSK3β is a key regulator of the ROS-dependent necrotic death induced by the quinone DMNQ
a Representative frames of two U87MG/GSK3β−/− cells re-expressing GSK3βWT-GFP treated with 30 µM DMNQ. Numbers indicate h before Δψm dissipation. Bar 16 µm. b Individual traces of cellular TMRM fluorescence (red) and of the nuclear/cytosolic fluorescence of GSK3βWT-GFP (green) in U87MG/GSK3β−/− cells re-expressing GSK3βWT-GFP. Cells were treated with 30 µM DMNQ for 24 h. c Individual traces of cellular TMRM fluorescence (red) and of the nuclear/cytosolic fluorescence of GSK3βWT-GFP (green) in untreated U87MG/GSK3β−/− cells, re-expressing GSK3βWT-GFP. d Immunoblot analysis of GSK3βWT-GFP levels in U87MG/GSK3β−/− cells treated with 30 µM DMNQ for the indicated times. Actin was used as loading control.
