Fig. 4: LINC00665 acts as a sponge for miR-379-5p.
From: LINC00665 promotes breast cancer progression through regulation of the miR-379-5p/LIN28B axis
a Representative FISH images indicating the subcellular localization of LINC00665 in MDA-MB-231 cells (green). Nuclei were stained with DAPI (blue). b Relative LINC00665 expression levels in the nuclear and cytoplasmic fractions of MDA-MB-231 cells. c The predicted binding site of miR-379-5p in LINC00665. The mut sequence contains an 8-base mutation in the miR-379-5p target seed region. d A dual luciferase reporter assay was performed to validate the interaction between miR-379-5p and LINC00665. e RIP analysis of endogenous AGO2 binding to RNA in MDA-MB-231 cells. IgG was used as the control. The LINC00665 and miR-379-5p levels were determined by RT-qPCR. f The miR-379-5p expression levels in LINC00665-depleted MDA-MB-231 cells (left) and LINC00665-overexpressing T47D cells (right), as well as in the corresponding control cells, were determined by RT-qPCR. ***P < 0.001.
