Fig. 4: Acer3 deficiency inhibits oxidative stress in hepatocytes in mice with NASH.

a Liver tissues from Acer3+/+ and Acer3−/− mice fed PEWD for 8 weeks were subjected to western blot analyses with the antibody against 4-HNE or β-Actin (a protein loading control). b and c Liver tissue sections from the above mice were stained with 4-HNE antibody to evaluate oxidative stress b and 4-HNE-positive hepatocytes were numerated c. Images in b represent results from five pairs of mice. ***P < 0.001.