Fig. 1: Deficiency of UCP2 protects against I/R-induced TIF. | Cell Death & Disease

Fig. 1: Deficiency of UCP2 protects against I/R-induced TIF.

From: UCP2-induced hypoxia promotes lipid accumulation and tubulointerstitial fibrosis during ischemic kidney injury

Fig. 1

a Representative images of human kidney sections with or without tubulointerstitial fibrosis with immunostaining for UCP2. Scale bars, 100 μm. b Representative images of mice kidney sections from sham, I/R, FAN, and AAN mice with immunostaining for UCP2. Scale bars, 50 μm. c Immunoblot analysis of UCP2 in kidney tissue from mice 6 weeks after I/R or sham laparotomy. Voltage-dependent anion channel (VDAC) served as the standard. *p < 0.05, ANOVA. d, e Renal dysfunction of I/R was determined in UCP2 wild-type (WT) or knockout (KO) mice 6 weeks after I/R or sham laparotomy. Blood urine nitrogen (BUN) d and serum creatinine (Scr) e were measured in sera; n = 7 per group. *p < 0.05, ANOVA. f Representative images of mice kidney sections from UCP2 WT or KO mice 6 weeks after I/R or sham laparotomy with H&E, masson and sirius red staining. Scale bars, 100 μm. g, h Fibrotic area g and total collagen content h in the kidneys from UCP2 WT or KO mice 6 weeks after I/R; n = 7 per group. *p < 0.05, ANOVA. i, j Immunoblot analysis of fibronectin i and collagen I j in kidney tissue from UCP2 WT or KO mice 6 weeks after I/R or sham laparotomy. Tubulin served as the standard. *p < 0.05, ANOVA. k Representative images of mice kidney sections from UCP2 WT or KO mice 6 weeks after I/R with immunostaining for fibronectin or collagen I. Scale bars, 50 μm.

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