Fig. 5: UCP2 promotes the stabilization of HIF-1α through regulation of mitochondrial respiration and oxygen content in tubular cells. | Cell Death & Disease

Fig. 5: UCP2 promotes the stabilization of HIF-1α through regulation of mitochondrial respiration and oxygen content in tubular cells.

From: UCP2-induced hypoxia promotes lipid accumulation and tubulointerstitial fibrosis during ischemic kidney injury

Fig. 5

a Representative images of mice kidney sections from UCP2 WT or KO mice 6 weeks after I/R with immunostaining for pimonidazole. Scale bars, 100 μm. b Immunoblot analysis for PHD2 in kidney tissue from UCP2 WT or KO mice 6 weeks after I/R or sham laparotomy. Tubulin served as the standard. *p < 0.05, ANOVA. c Representative images of UCP2 WT or KO PTCs treated with or without H/R with immunostaining for pimonidazole (red). Nuclei were counterstained with DAPI (blue). Scale bars, 10 μm. d Immunoblot analysis for PHD2 of UCP2 WT or KO PTCs treated with or without H/R. Tubulin served as the standard. *p < 0.05, ANOVA. e Representative traces show OCR in UCP2 WT or KO PTCs treated with or without H/R. Where indicted, oligomycin (1 μmol/L), FCCP (0.75 μmol/L), antimycin A, and rotenone (1 μmol/L) were added. f Summary data analyzed from three independent experiments shows OCR in UCP2 WT or KO PTCs treated with or without H/R. *p < 0.05, ANOVA.

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