Fig. 5: Depletion of LIS1 inhibits cell migration.

A549 cells transfected with siRNAs were subjected to the following analyses: a Western blotting analysis of the expression of NudCL2, myosin-9, and LIS1. β-actin was used as a loading control. b ImageJ software was used to quantify protein levels in (a). The relative amounts of myosin-9 and LIS1 compared with the control were calculated and shown. c Western blotting analysis of the expression of LIS1. d, e Transwell migration assays revealed the cell motility of control and LIS1-depleted cells. Scale bar, 200 μm. Cells that migrated to the undersides of the filters were counted. f–h The migration tracks of individual cells were traced by Imaris 9.1.2 software. Representative single-cell migration paths are shown. Euclidean distance and migration velocity were measured. i, j Wound healing assays showed collective cell migration at different time points. Dashed lines indicate wound edges. Scale bar, 200 μm. The distance of the wound was measured by ImageJ software. Quantitative data derived from at least three independent experiments are shown as the mean ± SD. n, sample size. *P < 0.05; **P < 0.01; ***P < 0.001. Student’s t-test.