Fig. 6: MAP3K7, a target gene of miR-143-3p, is regulated by CCDC144NL-AS1. | Cell Death & Disease

Fig. 6: MAP3K7, a target gene of miR-143-3p, is regulated by CCDC144NL-AS1.

From: Long non-coding RNA CCDC144NL-AS1 sponges miR-143-3p and regulates MAP3K7 by acting as a competing endogenous RNA in gastric cancer

Fig. 6: MAP3K7, a target gene of miR-143-3p, is regulated by CCDC144NL-AS1.

a Predicted binding targets for miR-143-3p binding target via Diana, miRDB, Targetscan, and miRtarBase. b The predicted binding sites between MAP3K7 and miR-143-3p. c. miR-143-3p mimics prominently reduced luciferase activity in MAP3K7-wild not in MAP3K7-mut in HEK293T cells. d Relative expression of MAP3K7 in GC tissues compared with adjacent normal tissues was analyzed from the TCGA database. e qRT-PCR was used to detect the MAP3K7 expression in 72 GC tissues and paired adjacent normal tissues. f, g Correlation analysis of the expression of CCDC144NL-AS1, miR-143-3p and MAP3K7 in 72 GC cases. h, i Relative mRNA and protein levels of MAP3K7 in BGC823 and SGC7901 cells transfected with miR-143-3p inhibitor, miR-143-3p mimics or their control groups. j, k Relative mRNA and protein levels of MAP3K7 in GC cells transfected with si-CCDC144NL-AS1 or its scrambled control. l, m Relative mRNA and protein levels of MAP3K7 in GC cells transfected with scrambled, si-CCDC144NL-AS1, miR-143-3p inhibitor or si-CCDC144NL-AS1 + miR-143-3p inhibitor. *p < 0.05, **p < 0.01, ***p < 0.001.

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