Fig. 1: The proliferative capacity of DM1 SSCs is impaired. | Cell Death & Disease

Fig. 1: The proliferative capacity of DM1 SSCs is impaired.

From: MBNL1 reverses the proliferation defect of skeletal muscle satellite cells in myotonic dystrophy type 1 by inhibiting autophagy via the mTOR pathway

Fig. 1: The proliferative capacity of DM1 SSCs is impaired.

a Morphology of the three cell lines (scale bar = 100 μm). b Representative immunofluorescence images of Pax7 (green), MyoD (red), and DAPI (blue) (scale bar = 50 μm) (n = 3). Arrows show the positive cells. c The proportion of proliferating SSCs (Pax7+MyoD+) in three cell lines (n = 3). d, e Proliferation was evaluated in three cell lines by immunofluorescence for Ki67 (green). Nuclei were stained with DAPI (blue) (n = 3). Scale bar = 50 μm. The bars represent the means ± SD; one-way ANOVA with Bonferroni post hoc test, *P < 0.05.

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