Fig. 2: The effect of 2F5 on DDX5 expression levels in different leukemia cell lines. | Cell Death & Disease

Fig. 2: The effect of 2F5 on DDX5 expression levels in different leukemia cell lines.

From: DDX5-targeting fully human monoclonal autoantibody inhibits proliferation and promotes differentiation of acute promyelocytic leukemia cells by increasing ROS production

Fig. 2

a Western blotting was performed to analyze the basal expression level in five leukemia cell lines and three corresponding healthy control cells. β-actin was used as an internal control. b DDX5 levels in NB4 and HL-60 cells are much higher than other six types of cells. *p < 0.05. DDX5 levels in three healthy control cells (neutrophil, PBMC and monocyte) are lower than in promyelocytic lymphoblastic and monocytic leukemia cells respectively. *p < 0.05. **p < 0.01. c–e DDX5 expression levels were detected by western blotting in NB4 cells 4 days after 20, 40, and 80 μM 2F5 treatment. *p < 0.05. f–h DDX5 expression levels were detected by western blotting in HL-60 cells 4 days after 20, 40, and 80 μM 2F5 treatment. *p < 0.05. i 80 μM 2F5 had no effect on DDX5 expression levels in CEM-C7 cells. j 80 μM 2F5 had no effect on DDX5 expression levels in Jurkat cells. k 80 μM 2F5 had no effect on DDX5 expression levels in THP-1 cells. l 80 μM 2F5 had no effect on DDX5 expression levels in neutrophils. m 80 μM 2F5 had no effect on DDX5 expression levels in PBMCs. n 80 μM 2F5 had no effect on DDX5 expression levels in monocytes.

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