Fig. 7: DIABLO/SMAC mimetics induce apoptosis via an autophagy-dependent caspase 8 activating platform.
From: SMAC mimetics induce autophagy-dependent apoptosis of HIV-1-infected macrophages
HIV-Mφ transfected with ATG5 siRNA (siATG5), ATG7 (siATG7), SQSTM1 (siSQSTM1), or scrambled shRNA (shNS) were treated with 2 µM LCL-161, 4 µM AT-406, 8 µM birinapant, or vehicle for 48 h. a Representative western blots of ATG7, ATG12–ATG5, FADD, MLKL, RIPK1, cleaved RIPK1 (cRIPK1), RIPK3, cleaved RIPK3 (cRIPK3), and SQSTM1, n=4. Densitometric analysis of blots is shown in Supplementary Fig. 2. b Cells were lysed and RIPK1 was immunoprecipitated (IP). The presence of ATG7, ATG12–ATG5, FADD, MLKL, cRIPK1, RIPK3, cRIPK3, and SQSTM1 and CASP8 in immunoprecipitates was assayed by western blot, n = 4. Densitometric analysis of blots is shown in Supplementary Fig. 3.
