Fig. 4: LINC00266-1 sponged miR-548c-3p and miR-548c-3p abolished the effect of LINC00266-1 on OS cell phenotypes.

a LINC00266-1 was distributed in both the cytoplasm and nuclei. b Eleven potential miRNAs binding LINC00266-1 were searched in the Diana and miRDB databases. Literature review showed that five miRNAs have been extensively explored. c MiR-548c-3p was downregulated in OS tissues. d Transfection efficiency of miR-548c-3p mimic and inhibitor. e MiR-548c-3p was upregulated in OS cells with LINC00266-1 knockdown. f LINC00266-1 was downregulated by overexpression of miR-548c-3p, and it was upregulated by knockdown of miR-548c-3p. g–i Dual-luciferase reporter assay showed that the B1 mutation failed to affect the luciferase activity in MT-1, MT-2 and MT-3 in MG63 and U2OS cells. j–l The proliferation, metastasis and apoptosis in MG63 cells were influenced by miR-548c-3p and LINC00266-1. m Relative levels of Bax, Ki67, MMP2 and MMP9 in MG63 cells were influenced by miR-548c-3p and LINC00266-1. n–p The proliferation, metastasis and apoptosis in U2OS cells were influenced by miR-548c-3p and LINC00266-1. q Relative levels of Bax, Ki67, MMP2 and MMP9 in U2OS cells were influenced by miR-548c-3p and LINC00266-1 (*p < 0.05. ^#p < 0.05, compared to sh-LINC00266-1 1# group).