Fig. 7: p21 regulates LC3II by interacting with LC3.
From: Autophagy is involved in the protective effect of p21 on LPS-induced cardiac dysfunction
a Representative western blot analysis of the upstream kinases associated with LPS-induced autophagy (left panel) and average fold change (right panel) in the indicated hearts (n = 6). b Representative western blot analysis of LC3, p62, and GAPDH (left panel) and average fold change (right panel) in NRCMs (n = 6). c CoIP assays to test the interaction between endogenous p21 and LC3 in the indicated groups of NRCMs (n = 3). d CoIP assays to test the interaction between exogenously expressed p21 and LC3 in HEK293T cells. FLAG-tagged p21 or HA-tagged LC3B were overexpressed in HEK293T cells (n = 3). *p < 0.05 versus Ctrl group. #p < 0.05 versus corresponding LPS group.
