Table 1 Primers used for real-time RT-PCR.
From: Autophagy is involved in the protective effect of p21 on LPS-induced cardiac dysfunction
Gene | Species | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|---|
TNFα | Mouse | ACTGAACTTCGGGGTGATCGGT | TGGTTTGCTACGACGTGGGCTA |
IL6 | Mouse | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC |
MCP1 | Mouse | TGGCTCAGCCAGATGCAGT | CCAGCCTACTCATTGGGATCA |
NOX2 | Mouse | TTCCAGTGCGTGTTGCTCGACA | TGGCGGTGTGCAGTGCTATCAT |
SOD2 | Mouse | CCGTCCGTGTCGCCGTCCTC | GCCGCGTGGTGCTTGCTGTG |
GAPDH | Mouse | ACTCCACTCACGGCAAATTC | TCTCCATGGTGGTGAAGACA |
TNFα | Rat | AGCATGATCCGAGATGTGGAA | TAGACAGAAGAGCGTGGTGGC |
IL6 | Rat | GTTGCCTTCTTGGGACTGATG | ATACTGGTCTGTTGTGGGTGGT |
MCP1 | Rat | AGTCGGCTGGAGAACTACAAGA | CTGAAGTCCTTAGGGTTGATGC |
SOD2 | Rat | AGCCTCCCTGACCTGCCTTA | CGCCTCGTGGTACTTCTCCTC |
NOX2 | Rat | TGAATCTCAGGCCAATCACTTT | AATGGTCTTGAACTCGTTATCCC |
GAPDH | Rat | GACATGCCGCCTGGAGAAAC | AGCCCAGGATGCCCTTTAGT |
