Fig. 5: Mithramycin’s attenuation of apoptotic mechanisms in neurons is cell death model-specific.
From: Mithramycin selectively attenuates DNA-damage-induced neuronal cell death
RCN were treated with 200 nM doxorubicin, 50 µM C2-ceramide, or 0.5 µM staurosporine +/−200 nM mithramycin. After 6 or 24 h, cells were harvested, equal amounts of whole-cell lysates were loaded onto an SDS-polyacrylamide gel and after electrophoretic separation and transfer to a membrane were incubated with antibodies against DNA-damage markers, phospho-p53 (S15) and its downstream proteins, PUMA and p21, as well as active caspase-3, its targets, PARP and Fodrin, and the synaptic marker PSD95. Protein levels (of bands indicated by arrows) were quantified by densitometry, normalized to β-actin signal and are presented as fold change compared with control levels. n = 3/groups for all groups. Data all represent mean ± SD. Significance assigned based on one-way ANOVA and Tukey post hoc test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs control; ^p < 0.05, ^^p < 0.01, ^^^p < 0.001, ^^^^p < 0.0001 vs cell death inducer alone.
