Fig. 6: Mithramycin’s effect on immunofluorescent markers of caspase activation in neurons is cell death model-specific.
From: Mithramycin selectively attenuates DNA-damage-induced neuronal cell death
RCN were treated with 200 nM doxorubicin, 50 µM etoposide, 50 μM C2-ceramide, or 0.5 μM staurosporine +/−200 nM mithramycin. After 6 h, cells were fixed and stained for neuronal markers (MAB2300, Neuro-Chrom™ Pan-Neuronal Marker), DAPI and cleaved Casp3 or cleaved PARP, imaged, and quantified. A representative field for each image at 63x magnification is shown. The Milli-MarkTM Pan-Neuronal Marker is not able to successfully stain those neurons who are close to the end stages of cell death (caspase activation); however, white arrows indicate cleaved Casp3/cleaved PARP cells that show a clear neuronal morphology. Quantification was performed on at least seven fields for each treatment. Data all represent mean ± SD. Significance assigned based on one-way ANOVA and Tukey post hoc test; *p < 0.05, ***p < 0.001, ****p < 0.0001 vs control; ^^p < 0.01, ^^^p < 0.001, ^^^^p < 0.0001 vs cell death inducer alone.
