Fig. 2: Experimental setup. Syngeneic Panc02 mouse models were established and divided into two groups. | Cell Death & Disease

Fig. 2: Experimental setup. Syngeneic Panc02 mouse models were established and divided into two groups.

From: High-dimensional single-cell analysis delineates radiofrequency ablation induced immune microenvironmental remodeling in pancreatic cancer

Fig. 2

RFA Treatments were initiated on the left side in RFA group when the tumor volume reached ~500 mm3, whereas the tumor on the right side did not receive any intervention. Tumors were harvested on day 3 after RFA treatment, and then digested and sorted for CD45+ immune cells. CD45+ immune cells were pooled together from three mice in each group and then subjected to scRNA-seq and TCR sequencing using a 10× Genomics pipeline.

Back to article page