Fig. 5: The effects of miR-3622a-3p and SALL4 on cell proliferation and apoptosis of CRC cells. | Cell Death & Disease

Fig. 5: The effects of miR-3622a-3p and SALL4 on cell proliferation and apoptosis of CRC cells.

From: MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4

Fig. 5

a–d The effect of SALL4 on CRC cell proliferation was evaluated by colony formation assay. The rescue experiment for miR-3622a-3p overexpression was performed by ectopic expression of SALL4 in DLD-1. The rescue experiment for miR-3622a-3p silencing was performed by inhibition of SALL4 in HCT116. e–h The promoting role of SALL4 in CRC cell proliferation was further validated by the EDU assay and the rescue experiments were performed. i–l Flow cytometric analysis was employed to explore the impact of SALL4 on apoptosis of CRC cells and the rescue experiments were performed. All data are from three independent experiments and are presented as the means ± SD (*p < 0.05, **p < 0.01).

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