Fig. 2: Overexpression of OSBPL3 promotes progression of CRC cells. | Cell Death & Disease

Fig. 2: Overexpression of OSBPL3 promotes progression of CRC cells.

From: RETRACTED ARTICLE: Upregulation of OSBPL3 by HIF1A promotes colorectal cancer progression through activation of RAS signaling pathway

Fig. 2: Overexpression of OSBPL3 promotes progression of CRC cells.

a Overexpression of OSBPL3 promotes cell growth in MTT assays. b Colony formation assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. c Soft agar assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. d Tumorigenesis in nude mice. Tumor volumes were measured on the indicated days. Data points are the mean tumor volumes ± SD. e The sections of tumor were under H&E staining or subjected to IHC staining using an antibody against Ki-67. f Transwell assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. g Three-dimensional morphogenesis assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. h Orthotropic transplantation assay. The representative gross images of the intestines and livers from different experimental groups are shown. Sections of the liver were stained with H&E. Arrows indicate the metastases in the intestines and livers(left). Box-scatter plots show the number of metastatic nodules in the liver as observed in each group (middle) and the overall survival time of each group (log-rank test, p < 0.05) (right).

Back to article page