Fig. 5: HIF1A regulated the expression of OSBPL3. | Cell Death & Disease

Fig. 5: HIF1A regulated the expression of OSBPL3.

From: RETRACTED ARTICLE: Upregulation of OSBPL3 by HIF1A promotes colorectal cancer progression through activation of RAS signaling pathway

Fig. 5: HIF1A regulated the expression of OSBPL3.

a Detection of the influence of Cocl2 on the expression of OSBPL3 in CRC. b Levels of OSBPL3 in HIF1A overexpressing cells were determined by quantitative RT-PCR and western blot. c Schematic depiction of the OSBPL3 promoter with three HIF1A binding sites, as indicated A, B and C, and the HIF1A B binding motif in the set A proximal promoter and its mutant containing altered nucleotides in set A (top). ChIP analysis of HIF1A binding to the OSBPL3 promoter in RKO cells. Primers against the −1721 to −1692 base pairs in the promoter region (set A) showed significant enrichment after normalization to the input control (bottom). RT-PCR experiments were performed. d Relative expression of a WT OSBPL3 promoter–driven luciferase reporter in Vector control or HIF1A-overexpression CRC cells (left) and the relative expression of WT or MUT OSBPL3 promoter–driven luciferase reporters in HIF1A -overexpression CRC cells (right). Error bars represent the mean ± SD of 3 independent experiments; **p < 0.01. e Colony formation assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. f Soft agar assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. g Transwell assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01. h Three-dimensional morphogenesis assay. Error bars represent mean ± SD from three independent experiments; **p < 0.01.

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