Fig. 1: p53-R273H promotes cell migration via downregulation of E-cadherin.
From: Hotspot mutant p53-R273H inhibits KLF6 expression to promote cell migration and tumor metastasis
Stable MCF-10A or HCC1806 cells expressing vector control (V) or p53-R273H (R273H) were subjected to western blot analyses for p53 and EMT markers as indicated (a), colony formation (b), transwell (c), or qPCR analyses (d). Stable MCF-10A or HCC1806 cells expressing p53-R273H were infected with lentivirus encoding E-cadherin, then subjected to western blot analyses (e) or transwell analyses (f). Scale bar = 100 μm. **P < 0.01. Results are presented as means ± SD from three independent experiments in triplicates. g The p53-R273H-suppressed genes coupled with p53-R273H ChIP-seq analyses, as described in the “Materials and Methods”, were represented in the heat map.
