Fig. 2: p53 hotspot mutants suppress KLF6 expression.
From: Hotspot mutant p53-R273H inhibits KLF6 expression to promote cell migration and tumor metastasis
Stable MCF-10A or HCC1806 cells expressing either p53-R273H, p53-R175H, or R248W were subjected to western blotting (a), qPCR (b), or transwell assays (c). MDA-MB-468, NCI-H1975, or MIA PaCa-2 cells were infected with lentivirus expressing either of two different shRNAs specific for p53 (shp53-#1 and shp53-#2), or a control shRNA (shGFP), followed by western blotting analyses (d), qPCR (e), or transwell assays (f). MCF-10A cells were infected with lentivirus expressing either of two different shRNAs specific for p53 (shp53-#1 and shp53-#2), or a control shRNA (shGFP), followed by western blotting or qPCR assays (g). MCF-10A stable cells expressing shp53-#1 (which targets the 3′ UTR of p53 mRNA) were infected with lentivirus encoding p53-R273H, prior to western blotting or qPCR assays (g). **P < 0.01, NS indicated no significance. Results are presented as means ± SD from three independent experiments in triplicates.
