Fig. 3: KLF6 suppresses p53-R273H-induced cell migration and tumor metastasis.

MCF-10A cells were infected with lentivirus expressing either of two different shRNAs specific for KLF6 (shKLF6-#1 and shKLF6-2#), or a control shRNA (shGFP), followed by western blotting or transwell assays (a). Stable MCF-10A or HCC1806 cells expressing p53-R273H were infected with lentivirus encoding KLF6, then subjected to western blotting (b) and transwell assays (c). Stable MCF-10A and HCC1806 cells expressing p53-R273H were infected with lentivirus encoding KLF6 wild-type (WT) or mutations (A123D, L169P, L217S, and C265Y), then subjected to western blotting (d) or transwell assays (e). H1299 stable cells expressing p53-R273H were infected with lentivirus encoding KLF6, then subjected to western blotting and transwell assays (f). H1299 stable cells, expressing mutant p53-R273H or a vector control (V), were infected with lentivirus encoding KLF6 or a vector control (Ctrl), tail-vain injected into female nude mice (ten mice per group). Mice were observed daily and sacrificed after 50 days. Lungs were dissected and fixed, and were inspected for metastatic nodules on their surface (g). Lungs were fixed, embedded in paraffin, sectioned, and stained by H&E for histological analysis, Scale bars = 1 cm (h). *P < 0.05; **P < 0.01. Results are presented as means ± SD from three independent experiments in triplicates.