Fig. 3: TLR4-induced pyroptosis in vitro relies on lncRNA-F630028O10Rik. | Cell Death & Disease

Fig. 3: TLR4-induced pyroptosis in vitro relies on lncRNA-F630028O10Rik.

From: TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury

Fig. 3

a LncRNA-F630028O10Rik levels in BV2 cells transfected with sh2203 or plvxF630 constructs. *p < 0.05 versus shNC; ###p < 0.001 versus plvxNC. b NLRP3, ASC, CASP-1 and GSDMD mRNA levels in plvxF630-expressing BV2 cells with/out Tka242 treatment. c, d IL-1β, IL-6, TNF-a and LDH mRNA levels in Tak242-treated BV2 cells with/out plvxF630 transfection. e, f Representative immunoblot and quantification of NLRP3, GSDMD, GSDMD-N and ASC proteins in each group. g CASP-1 representative immunoblot in Tak242-treated BV2 cells with/out plvxF630 transfection. h Representative IHF images showing in situ expression of NLRP3 (Red) and GSDMD (Green) in each group. Nuclei are stained with DAPI (Blue). Scale bar: 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001 versus Blank, #p < 0.05, ##p < 0.01, ###p < 0.001 versus LPS, &&p < 0.01, &&&p < 0.001 versus Tak242. Data are the mean ± SD from 3 independent experiments.

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