Fig. 5: LncRNA-F630028O10Rik acts as a sponge for miRNA-1231-5p to regulate Col1a1. | Cell Death & Disease

Fig. 5: LncRNA-F630028O10Rik acts as a sponge for miRNA-1231-5p to regulate Col1a1.

From: TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury

Fig. 5

a The relative expression of nuclear and cytosolic lncRNA-F630028O10Rik. b RNA-FISH images showing the subcellular localization of lncRNA-F630028O10Rik in BV2 cells. Scale bar: 50 μm. c CeRNA network predicted by miRanda (http://www.microrna.org/microrna/home.do) and RNAhybrid (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid). Red diamonds represent lncRNAs, red ellipses represent mRNAs, and blue V-shapes represent miRNAs. d The expression of predicted target genes in NC and lncRNA-F630028O10Rik-mus2203 cells. e, f RNA pull-down assay showing the physical relationship between lncRNA-F630028O10Rik and the predicted miRNAs (f) or target genes (e). g, h Relative luciferase activity of lncRNA-F630028O10Rik (g) and Col1a1 (h) promoter-driven reporter constructs. i The relative expression of Col1a1 in the blood samples of SCI patients and healthy controls. j Linear regression between lncRNA-F630028O10Rik and Col1a1 (r = 0.87, p < 0.001). *p < 0.05, **p < 0.01, ***p < 0.001. Data are the mean ± SD from 3 independent experiments.

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