Fig. 6: LncRNA-F630028O10Rik/miR-1231-5p/Col1a1 mediated pyroptosis via the PI3K/AKT pathway. | Cell Death & Disease

Fig. 6: LncRNA-F630028O10Rik/miR-1231-5p/Col1a1 mediated pyroptosis via the PI3K/AKT pathway.

From: TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury

Fig. 6

a MiR-1231-5p was downregulated by the specific inhibitor. **p < 0.01. b Col1a1 was upregulated by pCD-Col1a1. ***p < 0.001. c Expression levels of Col1a1 and pyroptosis-related genes in cells transfected with miR-inhibitor, pCD-Col1a1 and sh2203, with/out LY294002 treatment. d, e Expression levels of inflammatory kinases and LDH in cells transfected with miR-inhibitor, pCD-Col1a1 and sh2203, with/out LY294002 treatment. f, g Representative immunoblot and quantification of the indicated proteins in each group. h CASP-1 representative immunoblot in each group. i Representative immunofluorescence images showing in situ expression of GSDMD (Red) and NLRP3 (Green) in each group. Nuclei are stained with DAPI (Blue). *p < 0.05, **p < 0.01, ***p < 0.001 versus Control, #p < 0.05, ##p < 0.01, ###p < 0.001 versus sh2203, &p < 0.05, &&p < 0.01 versus sh2203+pCD-col1a1. Data are the mean ± SD from 3 independent experiments.

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